Journal: Frontiers in Cardiovascular Medicine

Article Title: Deficiency of Myeloid Pfkfb3 Protects Mice From Lung Edema and Cardiac Dysfunction in LPS-Induced Endotoxemia

doi: 10.3389/fcvm.2021.745810

Figure Lengend Snippet: Myeloid-specific Pfkfb3 deficiency attenuates LPS-induced inflammatory responses. (A) Representative images (left) and quantification (right) for immunohistochemical staining of the neutrophil marker Ly6G in lung sections of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 5). (B) Representative images (left) and quantification (right) for immunohistochemical staining of the macrophage marker Mac2 in lung sections of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 5). (C–E) qPCR analysis of the mRNA levels of Il1b (C) , Il6 (D) and Nos2 (E) in the lung of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 6). (F,G) ELISA analysis of Il1b (F) and Il6 (G) in serum of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 4). (H) NO levels in serum of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 3–4). All data are represented as mean ± SEM, ** P < 0.01 and *** P < 0.001 for Pfkfb3 WT vs. Pfkfb3 ΔMϕ (unpaired two-tailed Student's t test).

Article Snippet: After antigen retrieval with Antigen Unmasking Solution (H-3301, Vector Laboratories, Burlingame, CA, USA) at 98°C for 10 min, sections were blocked with avidin solution with 10% normal rabbit serum for 1 h at room temperature, and incubated in biotin blocking solution with primary antibodies against Mac2 (3 μg/mL, CL8942F, Cedarlane, Burlington, NC, USA), or Ly6G (3 μg/mL, 551459, BD biosciences, San Jose, CA, USA) at 4°C overnight.

Techniques: Immunohistochemical staining, Staining, Marker, Injection, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: Nature Communications

Article Title: Targeting hepatocytic TβRI ameliorates liver metastatic outcomes by revitalizing stem-like CD8 + Tex subsets

doi: 10.1038/s41467-025-65615-0

Figure Lengend Snippet: a Luminex showing the protein levels of inflammatory cytokines in tumor interstitial fluid of TβRI-hWT and TβRI-hKO mice ( n = 3 mice/group). Data are shown as a heatmap. b Experimental scheme for generating Gal-9 flox/flox Alb-Cre +/- (Gal9-hKO) mice. Gal-9 flox/flox Alb-Cre -/- and C57BL/6 J were used as Gal9-hWT mice. c Representative bioluminescent image of Gal9-hWT versus Gal9-hKO mice inoculated with TGFβ1 OE or TGFβ1 CTL -PAN02 cells in the portal vein on day 28 ( n = 6 mice/group). d Bioluminescence quantification of ( c ). e Statistical analysis depicting the percentages of CD39 + TOX + cells within CD8 + PD1 + CD44 + tumor-reactive T cells in the indicated groups. f Statistical analysis depicting the percentages of Tex subsets based on TCF1 and CX3CR1 within CD8 + PD1 + CD44 + tumor-reactive T cells. g statistical analysis depicting the TNF and IFNγ co-production by CD8 + CD44 + T subsets in the indicated groups upon ex vivo CD3/CD28 stimulation. h The diagram of experimental design. i Representative bioluminescent image of Gal9-hWT versus Gal9-hKO mice treated with IgG, αCD4, αCD8 or αPD-1( n = 5 mice/group). j Representative macroscopic image of ( i ). k Bioluminescence quantification of ( i ). l Statistical analysis depicting the percentages of Tex subsets based on Ly108 and CX3CR1 within CD8 + PD1 + CD44 + tumor-reactive T cells. Data are presented as the mean ± SD, and were analyzed with unpaired two-sided Student’s t test ( k ) or two-way ( d , e , f , g , l ) ANOVA with Tukey’s multiple comparison test. Consistent results were observed across three biological replicates in ( a , c–g , i–l ). Source data are provided as a Source Data file.

Article Snippet: For neutralization of Galectin-9, mice received 150 μg of αGal9 (RG9-1, BioXCell, BE0218) or rat IgG2b (LTF-2, BioXCell, BE0090) Isotype-control antibodies administration in 200 μL PBS.

Techniques: Luminex, Ex Vivo, Comparison

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: Galectin 9 , REA435 , 50 , 130-124-237 , PE (APC) , Miltenyi Biotec.

Techniques: Imaging